Bachelor’s / Master’s / Semester Thesis - Immobilization of Enzymes to Magnetic Nanoparticles

06.03.2025, Studentische Hilfskräfte, Praktikantenstellen, Studienarbeiten

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In this research project, genetically optimized E. coli strains expressing different extracellular high-value proteins will be used to establish different concepts of process intensification.

The focus of this work will be the development of a protocol for the immobilization of an industrial-relevant enzym to functionalized magnetic nanoparticles. Therefore, different parameters need to be investigated like:

- Effect of different buffers on immobilization
- Incubation times
- Particle to protein ratio
- Reusability
- Remaining activity after immobilization
- Application to relevant use cases

Your Tasks:
- Production & purification of the enzyme with E. coli
- Determination of enzymatic activity
- Functionalization of particles
- Analytics
- SDS Page
- Various assays
- Magnetic nanoparticles

Your Profile:
- Independent and structured way of working
- Experience with laboratory work
- Student in the field of biotechnology, biochemical engineering chemistry or similiar

Kontakt: Julian Galbusera; j.galbusera@tum.de; Chair of Bioseparation Engineering