Bachelor Thesis: Characterization of siRNA-mediated gene silencing and potential off-target effects in vivo and in vitro

19.10.2026, Abschlussarbeiten, Bachelor- und Masterarbeiten

We are looking for a motivated Bachelor's student for a 12-week thesis project starting January 11, 2027, in Molecular Vascular Medicine at TUM, Munich. The project focuses on siRNA-mediated gene knockdown in a mouse model of carotid atherosclerosis, including qPCR, RNA and protein isolation, Western blotting, and off-target analysis. Experience in molecular biology is desirable. Applications including CV to Jessica.pauli@tum.de.

Characterization of siRNA-mediated gene silencing and potential off-target effects in vivo and in vitro
We are looking for a highly motivated Bachelor's student to join our research team for a 12-week thesis project starting on January 11, 2027.
The project is embedded in an ongoing translational research collaboration and focuses on the molecular characterization of a siRNA-mediated knockdown of a protein-coding gene in a mouse model of carotid artery atherosclerosis.
Project Background
RNA-based therapeutics have emerged as a promising strategy for the treatment of various diseases. In our study, a target gene has been silenced using siRNA in vivo. Now, the knock-down efficiency, as well as additional analyses are required to investigate potential off-target effects at both the transcript and protein levels.
The Bachelor´s candidate will contribute to the molecular characterization of this intervention using a combination of gene expression and protein analysis techniques.
Your Tasks
• RNA isolation from mouse tissue samples (mainly kidney and liver for off-target effects)
• cDNA synthesis and quantitative real-time PCR (qPCR)
• Assessment of knockdown efficiency and potential off-target effects
• Expression analysis of additional candidate genes and biomarkers
• Protein isolation, mainly from liver and kidney tissue
• Establishment and optimization of the antibody
• Western blot analysis and data quantification
Optional Extension (Depending on Project Progress)
If time permits, the project may be expanded to include mechanistic studies in cell culture and histological analyses:
• Murine vascular smooth muscle cells (MOVAS)
• Primary human vascular smooth muscle cells
• Further characterization of the knockdown phenotype and downstream molecular effects
• Standard histological staining and immunohistochemistry (IHC) of kidney and liver tissue
Candidate Profile
• Bachelor's student in Biology, Biochemistry, Molecular Biotechnology, Biomedical Sciences, or a related field
• Strong interest in molecular biology and translational vascular research
• Previous laboratory experience in molecular biology is desirable
• Independent, reliable, and organized working style
What We Offer
• A hands-on research project in the field of Molecular Vascular Medicine
• Training in a broad range of standard molecular biology techniques
• Experience with qPCR, Western blotting, tissue processing, and potentially cell culture and histology
• Close supervision and integration into an international research environment
• Participation in a collaborative translational research project
Location: Campus Biederstein (Biedersteiner Str. 29), Munich, Germany
Duration: 12 weeks
Start Date: January 11, 2027

Please send your application including a CV to: Jessica.pauli@tum.de

Kontakt: jessica.pauli@tum.de