Master thesis – Genome editing in parasitic helminths

10.04.2025, Abschlussarbeiten, Bachelor- und Masterarbeiten

The chair of Infection Pathogenesis (Prof. Friederike Ebner) aims to understand the biology and immune evasion strategies of parasites, with the aim of developing intervention strategies and vaccines. In this master’s thesis, the student will be participating in developing a delivery method allowing that gene editing system (constructs of CRISPR-Cas, base editor or prime editing etc.) can be microinjected in unfertilized eggs of large roundworms.

Responsibilities:
The student will work on the isolation and purification of eggs from female Ascarids, larval embryonation, microinjection ,and the production of constructs for genomic editing in eggs of large roundworms.
The candidate will gain experience in microscopy, microinjections/micromanipulations, molecular genetics, primary cell culture methods, and parasitology.

Requirements:
We seek a highly motivated, structured, and reliable student of MBT, Biology, Biochemistry or similar disciplines. A successful candidate should already have a good knowledge of immunology and some experience in genetic engineering. A good command of English, both spoken and written, is essential.
If interested, please send an email including your earliest/latest possible start date, your transcript of records, and your CV to wei.liang@tum.de.

Kontakt: wei.liang@tum.de