Master’s thesis/ Bachelor’s thesis/Internship (Lehrstuhl für Bioseparation Engineering)

05.08.2025, Studentische Hilfskräfte, Praktikantenstellen, Studienarbeiten

Affinity-Based Separation of Lipocalin Protein from Natural Samples Using Antibody-Functionalized Resin

This project focuses on isolating one of the candidate proteins (Lipocalin proteins) with potential asthma-preventive activity, found in dust collected from cow farms. the functionalized chromatography resin with antibody will be tested to natural dust extracts from cow farms to isolate the candidate asthma-protective protein.

Research Objective

Isolate and identify candidate proteins in cow farm dust extract.
Assess the orientation of immobilized antibodies using Protein A and Protein L.
Analyze the antibody-depleted fraction.
Analytical Technique: SDS-PAGE, BCA assay, Western blot, HPLC, ELISA.


Profile:

Structured and independent work
Motivation to work as a team/ willing to learn
Master student in chemical engineering, biotechnology (IBT, MBT),Pharmaceutical --biotechnology biochemistry, chemistry, microbiology, biology or similar.


Start date: as soon as possible or September
Language: English
Ideal, but not required: Lab experience

Kontakt: t.khan@tum.de