Vortrag
Advanced Analytical Strategies for Exosome Characterization, Saccharide Profiling in Honey, and Nanomaterial-Based Glycopeptide Enrichment
Montag 04.05.2026, 11:15 - 11:45
Veranstaltungsort:
Gebäude CH-6, Seminarraum 36220, Fakultät Chemie, Lichtenbergstraße 4, 85748 Garching
Vortragender
Prof. Dr. Jeongkwon Kim
Topic: TUM IWC colloquium SuSe 2026
Teams meeting
https://teams.microsoft.com/meet/319656320670125?p=ZmePtzl0FtpemOgw0p
Meeting ID: 319 656 320 670 125
Pass code: 4GK9Kw3u
Colloquium Series in Analytical Chemistry and Water Chemistry
Summer Semester 2026
Advanced Analytical Strategies for Exosome Characterization, Saccharide Profiling in Honey, and Nanomaterial-Based Glycopeptide Enrichment
Prof. Dr. Jeongkwon Kim
Department of Chemistry, Chungnam National University, Daejeon, South Korea
Visiting Scientist, Helmholtz Munich, Germany
About Jeongkwon Kim:
Professional Experiences
2006.03–Current Chungnam National University, Chemistry, Assistant/Associate/Professor
2026.08–Current Visiting Scientist, Helmholtz Munich, Germany
2019.06–Current Director, CNU Chemistry Core Facility
2025.01–Current Editor-in-Chief, Analytical Science & Technology
2020.01–2023.12 Editor-in-Chief, Mass Spectometry Letters
2013.07–2014.07 University of Michigan Medical School, Visiting Scholar
2004.06–2006.02 Northeastern University, Barnett Institute, Senior Research Scientist
2002.04–2004.05 Pacific Northwest National Laboratory, Post-Doctoral Research Fellow
Research Area
–Exosome enrichment and analysis
–GC-MS analysis of various volatile compounds
–Characterization of proteins and carbohydrates using mass spectrometry
–Method development of matrix-assisted laser desorption/ionization
–LC-UV analysis of carbohydrates
Abstract:
This presentation discusses three major advancements in analytical chemistry from my laboratory. First, the focus is placed on the enrichment and characterization of extracellular vesicles (EVs), particularly exosomes. Size-exclusion chromatography (SEC) is evaluated, and polyethylene glycol (PEG)-based precipitation is optimized as a cost-effective, high-throughput alternative to commercial kits for serum and urinary EV analysis. Second, a comprehensive saccharide profiling method for honey is presented. By employing GC-MS with a two-step oximation/trimethylsilylation derivatization and a retention index-based peak assignment, precise identification and quantification of various saccharides are achieved, overcoming traditional analytical limitations. Finally, the synthesis and application of functionalized nanoparticles for glycoproteomics are introduced. Specifically, boronic acid-functionalized core-shell mesoporous silica and magnetic graphene oxide nanocomposites have been developed to selectively and efficiently enrich N-glycopeptides from complex biological samples. These integrated analytical approaches provide robust tools for biomarker discovery and food quality control.
Teams meeting
https://teams.microsoft.com/meet/319656320670125?p=ZmePtzl0FtpemOgw0p
Meeting ID: 319 656 320 670 125
Pass code: 4GK9Kw3u
Colloquium Series in Analytical Chemistry and Water Chemistry
Summer Semester 2026
Advanced Analytical Strategies for Exosome Characterization, Saccharide Profiling in Honey, and Nanomaterial-Based Glycopeptide Enrichment
Prof. Dr. Jeongkwon Kim
Department of Chemistry, Chungnam National University, Daejeon, South Korea
Visiting Scientist, Helmholtz Munich, Germany
About Jeongkwon Kim:
Professional Experiences
2006.03–Current Chungnam National University, Chemistry, Assistant/Associate/Professor
2026.08–Current Visiting Scientist, Helmholtz Munich, Germany
2019.06–Current Director, CNU Chemistry Core Facility
2025.01–Current Editor-in-Chief, Analytical Science & Technology
2020.01–2023.12 Editor-in-Chief, Mass Spectometry Letters
2013.07–2014.07 University of Michigan Medical School, Visiting Scholar
2004.06–2006.02 Northeastern University, Barnett Institute, Senior Research Scientist
2002.04–2004.05 Pacific Northwest National Laboratory, Post-Doctoral Research Fellow
Research Area
–Exosome enrichment and analysis
–GC-MS analysis of various volatile compounds
–Characterization of proteins and carbohydrates using mass spectrometry
–Method development of matrix-assisted laser desorption/ionization
–LC-UV analysis of carbohydrates
Abstract:
This presentation discusses three major advancements in analytical chemistry from my laboratory. First, the focus is placed on the enrichment and characterization of extracellular vesicles (EVs), particularly exosomes. Size-exclusion chromatography (SEC) is evaluated, and polyethylene glycol (PEG)-based precipitation is optimized as a cost-effective, high-throughput alternative to commercial kits for serum and urinary EV analysis. Second, a comprehensive saccharide profiling method for honey is presented. By employing GC-MS with a two-step oximation/trimethylsilylation derivatization and a retention index-based peak assignment, precise identification and quantification of various saccharides are achieved, overcoming traditional analytical limitations. Finally, the synthesis and application of functionalized nanoparticles for glycoproteomics are introduced. Specifically, boronic acid-functionalized core-shell mesoporous silica and magnetic graphene oxide nanocomposites have been developed to selectively and efficiently enrich N-glycopeptides from complex biological samples. These integrated analytical approaches provide robust tools for biomarker discovery and food quality control.
Veranstalter
Lehrstuhl für Analytische Chemie und Wasserchemie
Ansprechpartner
Frau Dorothea Skottke


