I present rather unusual suspects in the world of gene regulation – transfer RNA fragments (tRFs). Such fragments have previously escaped detection or have been generally ignored as noise due to low count numbers in of small RNA libraries. While the focus in the analysis of such libraries has been primarily on microRNAs (miRNAs), recent studies have reported findings of tRFs across a range of organisms. We had previously described age-related dynamics of miRNA loading into different RNA- induced silencing complexes (RISC) of Drosophila melanogaster using small RNA- Seq data. Here I focus on tRFs found in the same transcriptome libraries, and their structural and functional features that make these fragments surprisingly similar to miRNAs. We found that Drosophila tRF reads mapped to tRNA genes for all 20 amino acids and detected fragments of both nuclear and mitochondrial tRNAs. Following the similarities with miRNAs and based on complementarity with conserved Drosophila genome regions we described potential seed sequences found in the most abundant tRFs. Further, we identified their possible targets with matches in the Drosophila melanogaster 3'UTR regions. Strikingly, these potential target genes of the most abundant tRFs show significant Gene Ontology enrichment in development and neuronal function. This observation suggests that involvement of tRFs in the RNA interfering pathway may play a role in brain activity or brain changes with age.